Nonetheless, western blot sensitivity is dependent on efficiency of blotting or transfer, retention of antigen during processing and the final detection/amplification system used. Protein blotting has been evolving constantly, since its inception, and now the scientific community is faced with a multitude of ways and means of transferring proteins ( 10). It offers the following specific advantages (a) wet membranes are pliable and are easy to handle compared to gels, (b) easy accessibility of the proteins immobilized on the membrane to different ligands, (c) only small amount of reagents are required for transfer analysis (d) multiple replicas of a gel are possible (e) prolonged storage of transferred patterns, prior to use, becomes possible and (f) the same protein transfer can be used for multiple successive analyses ( 7– 9). This procedure ( 1, 2) is a powerful tool to detect and characterize a multitude of proteins, especially those proteins that are of low abundance. Protein transfer with subsequent immunodetection has found wide application in the fields of life sciences and biochemistry. The utility of the high resolving power of SDS PAGE ( 5) was limited in purpose, owing to the fact that the separated proteins in the gel matrix were difficult to access with molecular probes, until the advent of protein blotting. (C) Primary antibody binding to a specific band on the blot (D) Secondary antibody conjugated to an enzyme (alkaline phosphatase or horse radish peroxidase) binding to primary antibody (E) color development of specific band (Reproduced from Ref 10 with permission from Elsevier). The bands shown are hypothetical (B) Exact replica of SDS PAGE gel obtained as a blot following Western transfer. (A) Unstained SDS PAGE gel prior to Western blot. Schematic representation of Western blotting and detection procedure Dot blotting refers to the analysis of proteins applied directly to the membrane rather than after transfer from a gel. The subsequent employment of antibody probes directed against the membrane bound proteins (immunoblotting) has revolutionized the field of immunology ( Fig. The blotted proteins form an exact replica of the gel and have proved to be the starting step for a variety of experiments. The term ‘western blotting’ was coined to describe ( 5) this procedure to retain the ‘geographic’ naming tradition initiated by Southern’s paper ( 3) ( see Chapter 3). Protein blotting evolved from DNA (Southern) blotting ( 3) ( see Chapter 1) and RNA (Northern) blotting ( 4). Nucleic acids are routinely transferred from agarose gels, to a membrane support, through capillary action (Southern blotting). Proteins that are resolved on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) gels are typically transferred to adsorbent membrane supports under the influence of an electric current in a procedure that is known as western blotting (WB) or protein blotting ( 1, 2) ( see Chapters 2 and 3). The transfer of proteins or nucleic acids to microporous membranes is referred to as “blotting” and this term encompasses both “spotting” (manual sample deposition) and transfer from planar gels.
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*rup* → *rap*: As in, “ Cor rapt government” and “A dis rapt ending.” Here are other words you could use: ab rapt (abrupt), inter rapt (interrupt), cor raption (corruption), unsc rapulous (unscrupulous), rapture (rupture) and dis raptive (disruptive).Rep* → Rap*: We can replace the “rep” sound in words with “rap” to make some sneaky rap-related puns, as in: “A false raport” and “Banana rapublic.” Other word that could be used are: raproach (reproach), rapresent (represent), raprieve (reprieve) and raprimand (reprimand).Wrap → Rap: As in, “It’s a rap” and “Keep under raps” and “ Rap it up” and “ Rapped around your little finger.”.Trap → Rap: As in, “Tourist rap” and “The parent rap” and “Shut your rap” and “Fall into a rap.”.Snap → Rap: As in, “A rap decision” and “ Rap happy” and “ Rap out of it” and “ Rap to it” and “ Rap, crackle and pop” and “Make it rappy.”.Gap → Rap: As in, “Bridge the rap” and “Generation rap” and “Mind the rap.”.Cap → Rap: As in, “A feather in your rap” and “ Rap in hand” and “If the rap fits, wear it” and “Put on your thinking rap” and “To rap it all off…”.Rap: These rap-related phrases are general enough to use as puns: “Beat the rap” and “A rap over the knuckles” and “A bad rap.” Note: a bap rap is a bad reputation.Rap: Rap is a very popular music genre that is characterised by rhythmic rhymes.Shock → Rock: As in, “ Rock and awe” and “ Rock horror” and “A short, sharp rock to the system” and “Sticker rock.”.Knock → Rock: As in, “Don’t rock it til you’ve tried it” and “I get rocked down, but I get up again” and “ Rock ’em dead” and “ Rock back a few” and “ Rock heads together” and “ Rock it off” and “ Rock it out of the park” and “ Rock on wood” and “ Rock your socks off.”.Clock → Rock: As in, “ Anti- rockwise” and “Around the rock” and “Regular as rockwork” and “Beat the rock” and “Race against the rock” and “Running like rockwork.”.Chock → Rock: As in, “ Rock a block” and “ Rock full.”.Chalk → Rock: As in, “Different as rock and cheese” and “ Rock it up to experience.”.Block → Rock: As in, “Mental rock” and “New kid on the rock” and “Chip off the old rock.”. Rock: Rock is a mainstream music genre.Dig → Gig: As in, “ Gig your own grave” and “ Gig your heels in” and “ Gig up the dirt” and “A gig for victory.”.Big → Gig: As in, “A gig ask” and “ Gig shot” and “Earning gig bucks” and “Look at the gig picture” and “Bright lights, gig city” and “Make it gig” and “No gig deal” and “Open your gig mouth.”.Spot → Spotify: As in, “A blind spotify” and “Got a soft spotify for” and “That really hit the spotify” and “In a tight spotify” and “ Spotify the difference.” Note: Spotify is a music provider.Hop → Hip Hop: As in, “ Hip hop to it” and “ Hip hop, skip and jump” and “ Hip hopping mad.” Note: hip hop is a popular music genre.Can you → Kanye: As in, “ Kanye get it done by 5pm?” and “ Kanye think about it?” Note: This is a reference to Kanye West, a famous rapper.Apparatus → Opera-tus: As in, “A complicated opera-tus.”.Tenner → Tenor: As in, “That’ll cost you a tenor.”.Hand → Band: As in, “ Bands on deck” and “Bite your band off” and “Bound band and foot.”.Grand → Band: As in, “Delusions of bandeur” and “ Band theft auto” and “ Band slam” and “A band slam” and “The band canyon.”.Brand → Band: As in, “ Band new” and “A band new ballgame” and “A band new day” and “ Band spanking new.”.Sand → Band: As in, “Draw a line in the band” and “The bands of time.”.Land → Band: As in, “ Band ahoy!” and “ Band of hope and glory” and “ Band of make-believe” and “In the band of the living.”.Bend → Band: As in, “ Band over backwards” and “ Band the rules” and “ Band your ear.”.Soon → Tune: As in, “A fool and their money are tune parted” and “As tune as my back is turned” and “As tune as possible” and “Get well tune” and “ Tuner or later” and “Too much too tune.”.If you know of any puns about music that we’re missing, please let us know in the comments at the end of this page! Without further ado, here’s our list of music puns: Music Puns ListĮach item in this list describes a pun or a set of puns that can be made by applying a rule. You might also be interested in our art puns or party puns. While we’ve made this list as thorough as possible, it is specific to music. We’ve done our best to cover this broad scope with music puns that span from composers to musicians to musical terminology. We hear music not only in songs, but also in advertisments, movies, stories and tv shows (even wrestlers have theme songs!). Music is a huge and deeply ingrained part of our culture. Welcome to the Punpedia entry on music puns! □ □ □ □ The warm colors of the hardwood floors and classic wood furniture create a beautiful contrast with the blue accents in this Park at Brushy Creek dining room. This bathroom at Larkspur is completely transformed with royal blue shower curtains and a matching painting. Brilliant shades of blue and orange work together to make this bedroom in the Meadows at Shadow Creek feel warm and welcoming. These matching end tables in the Addison do the job on their own.Ĭontrasting colors are great in spring. It doesn’t take much to make your space feel more in tune with the season. The decor in this Addison living room shows how you can use just a few small elements scattered throughout the space to create a unified spring look.Ī blue-and-white bedspread as well as matching pillows transform your bedroom into a spring oasis. Shades of blue and gray go great together! With a few simple colors, this Larkspur living room gains a classic look. This living room at the Hills of Bear Creek is another great example of how repainting one or two walls can transform your living space. A single painting and this brilliant blue afghan do the trick, as shown here at the Eastwood at Riverside. It doesn’t take much to welcome spring into your bedroom. The simple addition of a few blue decor pieces give the Eastwood at Riverside kitchen a light, spring-like atmosphere. The cabinets at the Park at Brushy Creek are perfect for showing off your blue spring decor. The matching quilt and throw pillows in this Larkspur bedroom are all you need to relax and enjoy the spring season. Light blue candles are a great touch in this Scottsdale Crossing dining room. For example, the paintings and throw pillows in this Griffin living room tie the design together. Think about how you can make the “background” and “foreground” in your home work together. It doesn’t take a lot of effort to bring a springtime atmosphere into your home! This bathroom at the Griffin shows how less is sometimes more. This blue tile backsplash in the Addison kitchen is great for any season and feels right at home in spring. This bright blue front door at the Addison makes a bold statement by welcoming spring and making your guests feel right at home. These baby-blue table settings at Scottsdale Crossing give the dining room a light spring vibe without overdoing it. Plus, that blue-and-gray rug really ties the room together. Repainting a single wall can transform your space, just like this bright shade of aquamarine does for this Arbors at Lakeline living room. From major to minor decor changes, these blue home decor ideas will put your home in full bloom this spring. Also, for many homeowners, it’s an opportunity to introduce the colors of the season into the home decor. For anyone looking for budget living room makeover ideas, it's worth knowing that even a small detail, such as a painted door frame, can boost your space, with very little spend.The arrival of spring means longer days as well as a chance to do some much-needed spring cleaning. They needn't just be used to paint walls you can revamp furniture and even flooring with paint. Paint tricks are, comparably, in expensive but can be seriously transformative. How can I decorate my home on a low budget?ĭecorating a home on a budget is best done with paint. Using adventurous, subtle or bold paint ideas to pick out accent colors, to tie open-plan spaces together and even to create a color thread from one room to another is a simple technique for boosting your home decor. The easiest home decor ideas are all about paint. And, of course, there's no reason not to adorn walls with beautifully styled shelves, too. One is the color of your boundaries, whether walls or fences: paint these a dark color and they will visually recede, while providing a beautiful backdrop for planting. Outdoor living room ideas and outdoor dining ideas should be no exception to home decor rules, and while you might not introduce every aspect of interior design to your exterior, there are a number of elements that can work really successfully. Credits to original model component designers and creators are. I have assembled individual models together using Sweet Home 3D, SketchUp and of course Sketchfab. Most of the 1/6th scale figures sell between $199 and #399, so scale that up for the Millennium Falcon and Tie-FIghter and you better have deep pockets, and lots of space at home, if you plan on getting these. This model is used for Mixed Reality / education and design workshops and is focussed on the cockpit of the Millennium Falcon using a myriad of models created by different individuals. The company is back with a new DeLorean and a new Marty McFly from Back to the Future II.Īlso to celebrate 2015 is the year Marty McFly has travel to in Back To the Future Part II, the new prototypes of the 1/6th scale Marty McFly and DeLorean Time Machine!Īnd the craziness continues as the company showed off its entire line of 1/6th scale Age of Ultron figures, including the newly unveiled Maria Hill, and Ant-Man figures. Like you've never seen it before Make yourself at home at the controls of the fastest hunk of junk in the galaxy with this 360-degree photo of the Millennium Falcon’s cockpit If you’re. Last year, Hot Toys blew our minds (and our budget for the year) when they unveiled a 1/6th scale DeLorean from Back to the Future. In addition to the large spacecraft from the epic Star Wars saga, the newly unveiled prototypes of the 1/6th scale Luke Skywalker in Stormtrooper diguise, Princess Leia, Return of the Jedi Boba Fett, and a 1/4th scale Stormtrooper are also a big attraction for Star Wars fans! Wait… did they say Imperial Tie Fighter? As in a 1/6th scale tie-fighter? Yup. Ever wanted to step inside the Millennium Falcons cockpit Check out the seats Look through the windows See what needs some paint Now you can. The new installment of the Star Wars series will arrive in cinemas in December, and to anticipate for the continuation of the Star Wars legacy, Hot Toys has created an extremely detailed 1/6th scale Millennium Falcon Cockpit along with an imperial TIE Fighter! RF 2K84285 Blurred Millennium Falcon and Star Wars spaceship from an inside view, Stock Photo. How big is this piece? Consider that the four figures inside the cockpit are indeed 12-inches tall, and that will give you an idea of how big this piece of Star Wars ephemera really is. If you thought Hot Toys lost their marbles when they unveiled a 1/6th scale DeLorean last year at San Diego Comic Con, the company just got bigger with it’s 1/6th scale Millennium Falcon cockpit. with much-discussed amateurism rules Spanish seasoning Have Paycheck abbr. As a title designer Danny has collaborated with top film directors and producers to create some of the most. He won an Emmy for the Six Feet Under title sequence and has 2 Emmy nominations, as well as industry awards in every major design show. " Elizabeth of cosmetics Question that introduces doubt Something a judge might show "It's nothing," in Spanish Where some unsolicited advice comes from Course challenge Black _ "Cool, dude!" Prone Where the Noah's Ark story is thought to have occurred, today Essential work One might be educated Unsettle Subject of David Remnick's "King of the World" "All Eyez on Me" rapper It's thought to ward off bad energy H One making good points in the classroom? Something no two people can be _ buco 17-Across offering Spotted Kind of muscle contraction Tender union? Cousins of crew cuts Fit Twinkle San Rafael's county Director Sergio Struck dumb One face of the moon Bolster Identifier seen in the "Six Feet Under" title sequence Actress Alexander of "Get Out" 2010's _-Frank Act Name for a Dalmatian, perhaps Card games are played in it Grp. Item seen in the Six Feet Under title sequence SOLUTION: TOETAG Did you find the solution of Item seen in the Six Feet Under title sequence crossword clue Check the other crossword clues of LA Times Crossword FebruAnswers. Danny Yount is an american designer of film and television title sequence and promotional campaigns. Confront, in slang Some origin stories "Rumors are carried by _, spread by fools and accepted by idiots" (old saying) Moles are found in it 1991 platinum debut album by a female singer Fantastic voyage Collaborative resource React to a baby, maybe Promulgate Food often served with plastic grass Onetime Mughal capital Dirt farm? Ran Army _ Befuddled Ushered Medalla material "Ain't gonna happen" Country rocker Steve Small-batch publication It's often framed Treatment for jet lag One of the Scooby-Doo gang Elder brother of Moses Like talk, they say Pentagon inits. Search for crossword clues found in the Daily Celebrity. You can easily improve your search by specifying the number of letters in the answer. Below are all possible answers to this clue ordered by its rank. We think the likely answer to this clue is TOETAG. More answers for DecemProducer of inflation Ranking no. This chapter focuses on the main title sequences of recent television serials commissioned by the North American premium cable channel Home Box Office. Answers for Item seen in the 22Six Feet Under22 title sequence crossword clue, 6 letters. The crossword clue Item seen in the 'Six Feet Under' title sequence with 6 letters was last seen on the February 15, 2020. Jazwares’ mission is to entertain through the creation of innovative product. Jazwares, which is headquartered in Sunrise, FL, has offices around the world and sells in over 100 countries. Browse 2 zoe zebra stock photos and images available, or start a new search to explore more stock photos and images. Zoe Zebra, the Postmans Daughter pictures and photo gallery - Check out just released Zoe Zebra, the Postmans Daughter pics, images, clips, trailers. With over 20 years of design, development, and manufacturing expertise, Jazwares is a company with a progressive focus on identifying new trends and transforming them into high-quality products for consumers of all ages. Jazwares, a subsidiary of Alleghany Capital Corporation, specializes in consumer products including toys, plush, collectibles, musical instruments, and consumer electronics. There are so many Mini Buggies to collect! Which ones will you find? Peppa’s Free-Wheelin’ Friends Mini Buggies makes the perfect gift for birthdays, treasure boxes, basket stuffers, or just because! Boys and girls all love Peppa! Search more high quality free transparent png images on and share it. Peppa Pig Free-Wheelin’ Friends Mini Buggies characters from the hit show, Peppa Pig! See the popular characters in their awesome vehicles! 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There you go! Now when you know how to delete Viber messages, you can easily get rid of unwanted personal conversations or group chats. Once the backup is restored, you will be notified so that you can remove your iPhone. Go to its WhatsApp Transfer > Viber > Restore option and just select the backup file that you want to get back.Īfter selecting the backup file, click on the “Start” button and wait as your Viber chats would be restored to your phone. To restore an existing backup, connect your iPhone to the system and launch the MobileTrans application. Now, go to its WhatsApp Transfer module and select Viber from the sidebar.įrom here, click on the “Backup” button and just wait for a while as the application would take a complete backup of your Viber chats. Using a working cable, connect your phone to the computer and launch MobileTrans on it. Here’s how you can backup and restore Viber chats using MobileTrans with a single click. It can backup your Viber data from your iOS device to the computer and you can later restore the backup to the same or any other iOS device. with a single click, simply take the assistance of MobileTrans - WhatsApp Transfer. To backup your Viber messages, contacts, attachments, etc. If you use Viber to chat with your friends or for your work, then it is important to maintain its backup. You may also be interested in How to Find/Delete/Restore Viber Backup in Google Drive? The selected message would be removed and a prompt stating that the message has been deleted will be displayed instead. From the available options, tap on “Delete for everyone” so that the message would be erased from the chat. You can also tap and select multiple messages to delete. Tap and hold the message that you wish to delete to get more options. Firstly, just launch Viber and go to the conversation or group where you have sent a message by mistake. To learn how to delete Viber messages that you didn’t want to send, follow these steps: Once you have removed the message, the actual content would be deleted and the app will display a prompt that the message has been deleted. If you have accidentally sent a message to someone on Viber, then you can delete the message entirely from the conversation. Part 3: How to Delete Viber Messages You Didn't Want to Send? Tap on the “Clear Message History” option and confirm your choice to delete the entire message history on Viber. Now, browse to its Settings > Calls and Messages. Launch Viber and go to its menu from the side. As several options would appear on a pop-up, tap on “Delete” and confirm to get rid of the conversation. Android users can just launch Viber and hold the chat that they wish to delete. Tap on the Delete option from here and confirm your choice. If you have an iPhone, then just launch Viber and slide the chat that you wish to get rid of. From here, choose to delete the message and it will be disappeared from the thread. You can just select multiple messages as well. Long press and hold a message to get various options. Launch Viber and open any chat on which you have the messages that you wish to delete. You can delete certain messages, clear an entire chat, or delete the entire Viber message history as well. Part 2: How to Delete Viber Messages from a Conversation?īesides group chats, we can also delete Viber messages on individual conversations as well. Scroll all the way down, tap on the “Leave and Delete” option, and confirm your choice. If you want, you can open the group chat as well and slide from the right panel to get its more options. Just tap on the “Delete Chat” and subsequently select the option to “Leave and Delete” the group. This will display various options for the group. Simply launch Viber on your phone and tap and hold the group icon from its home that you wish to leave. Just tap on the option of your choice and delete the message for just you or everyone in the group.ġ.2 Delete Viber messages and leave the group This will give you an option if you wish to delete a message for yourself or everyone. You can also select multiple messages and tap on the Delete option. Tap and hold the messages that you wish to get rid of and select the “Delete” option. Launch Viber on your smartphone and open any group where you have earlier sent the message. 1.1 Delete Viber messages without leaving a group You can either just delete a message sent by you to a group or leave and delete the group as well. Therefore, before I teach you how to delete Viber messages on a personal chat, I will cover the same for groups. Part 1: How to Delete Viber Group Messages?Īpart from individual chats, Viber also lets us create groups as well. Part 4: Bonus Tip: Take a Backup for Viber Messages AMD Smart Access Memory (Resizable BAR) Guide.NVIDIA Resizable BAR : What You Need To Know!.NVIDIA Reflex Guide : How To Reduce System Latency!.Control Craft 3 : How To Get This Game For FREE!.Royal Defense : How To Get This Game For FREE!.Touch ‘n Go eWallet Is Now Back On Apple App Store!.He continues to devote countless hours every day writing about tech, medicine and science, in his pursuit of facts in a post-truth world. Adrian Wong has been writing about tech and science since 1997, even publishing a book with Prentice Hall called Breaking Through The BIOS Barrier (ISBN 978-0131455368) while in medical school. Name : Adrian Wong Bank Transfer : CIMB 7064555917 (Swift Code : CIBBMYKL)ĭr. Support my work through a bank transfer / PayPal / credit card! That’s it! It is now added to your Epic Games account, and you can download and install it anytime after this. 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(Signup button is located at the top of right) Once you there, You need to create an account by clicking on Signup button. version: '3'services: plex: image: plexinc/pms-docker:plexpass restart: unless-stopped security_opt: - seccomp:unconfined runtime: nvidia ports: - 32400:32400/tcp - 33400:33400/tcp - 3005:3005/tcp - 8324:8324/tcp - 32469:32469/tcp - 1900:1900/udp - 32410:32410/udp - 32412:32412/udp - 32413:32413/udp - 32414:32414/udp environment: - TZ=America/Halifax - PLEX_CLAIM=xxxx - ADVERTISE_IP= - NVIDIA_VISIBLE_DEVICES=all - NVIDIA_DRIVER_CAPABILITIES=compute,utility,video hostname: xxxx volumes: - /mnt/xxxx/infrastructure_configs/plex_config:/config - /mnt/xxxx/infrastructure_configs/plex_transcode:/transcode - /mnt/xxxx/TVShows:/TVShows - /mnt/xxxx/TVShowsArchive:/TVShowsArchive - /mnt/xxxx/Movies:/Movies - /mnt/xxxx/KidsMovies:/KidsMovies - /mnt/xxxx/Music:/Music - /mnt/xxxx/Photos:/Photos devices: - /dev/nvidia0:/dev/nvidia0 - /dev/nvidiactl:/dev/nvidiactl - /dev/nvidia-caps:/dev/nvidia-caps - /dev/nvidia-modeset:/dev/nvidia-modeset - /dev/nvidia-uvm:/dev/nvidia-uvm - /dev/nvidia-uvm-tools:/dev/nvidia-uvm-tools labels: - "traefik.enable=true" - ".rule=Host(`xxxx`)" - ".entrypoints=websecure" - ".tls=true" - ".tls.certresolver=lets-encrypt" - ".rver. I don't know why the code block is unformatting everything. I needed to add the nvidia devices or I would have to run in priviledged mode. OK I got it working assign from needing nvidia-container-runtime and disabling cgroups in /etc/nvidia/settings.json(?). NVIDIA_DRIVER_CAPABILITIES=compute,utility,video Your docker compose file seems fine (you can remove the 'devices' section), although you are not using the plexpass image, and for the host configuration just follow: Kernel modules: nvidiafb, nouveau, nvidia_drm, nvidiaĮven with the latest nvidia-headless-495 driver package I can't seem to make it work. Memory at f0000000 (64-bit, prefetchable) Įxpansion ROM at 000c0000 Ĭapabilities: Power Management version 3Ĭapabilities: MSI: Enable+ Count=1/1 Maskable- 64bit+Ĭapabilities: Express Legacy Endpoint, MSI 00Ĭapabilities: Latency Tolerance ReportingĬapabilities: Advanced Error ReportingĬapabilities: Vendor Specific Information: ID=0001 Rev=1 Len=024 Ĭapabilities: Secondary PCI Express Memory at e0000000 (64-bit, prefetchable) Memory at f6000000 (32-bit, non-prefetchable) GP106 įlags: bus master, fast devsel, latency 0, IRQ 134 mnt/xxxx/TVShowsArchive:/TVShowsArchiveĬould someone who may already have this working validate if this is correct? Would you know what linux packages need to be installed? # lspci -v -s $(lspci | grep VGA | cut -d" " -f 1)Ġ1:00.0 VGA compatible controller: NVIDIA Corporation GP106 (rev a1) (prog-if 00 ) mnt/xxxx/infrastructure_configs/plex_transcode:/transcode mnt/xxxx/infrastructure_configs/plex_config:/config ADVERTISE_IP= - NVIDIA_VISIBLE_DEVICES=all #Enables NVIDIA devices I need to know what driver packages I will need to load to make it work in ubuntu server.This is my proposed config. I am planning on using this docker compose on my ubuntu docker server. Please go to the relevant subreddits and support forums, for example: Build help and build shares posts go in their respective megathreads No referral / affiliate links, personal voting / campaigning / funding, or selling posts Welcome to /r/Plex, a subreddit dedicated to Plex, the media server/client solution for enjoying your media! 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